October 15, 2013
Minnesota Beef Expo
Official ID Reminder
The Minnesota Beef Expo will be held October 17 - 20 on the State Fairgrounds. Please remember that all exhibition cattle need to be officially identified. Visit our cattle and bison official ID web page to view acceptable ID. Cattle coming into Minnesota from another state must also be accompanied by a Certificate of Veterinary Inspection and a permit number issued by the Board.
The Board of Animal Health offers free official ID to Minnesota livestock producers and veterinarians. Call 651-296-2942 or fill out the on-line tag order form to place your order.
Again this year we are teaming up with the Minnesota Department of Agriculture (MDA) to staff a booth at the Expo. Stop by for free livestock inventory worksheets, pocket-sized record books, and to learn about MDA's farm transition program and livestock investment grant opportunities.
New Weapon Against Killer of Baby Pigs
Submitted by the Veterinary Diagnostic Laboratory
All of a sudden, an outbreak of diarrhea appears in a herd of hogs.
Any number of infectious agents could be sickening the pigs, but if it's porcine epidemic diarrhea virus (PEDv), it will, if unchecked, wipe out all piglets under two weeks old.
Quick action is necessary to defend against PEDv, which has recently entered the Western Hemisphere and struck herds in at least 17 states, including a handful of cases in Minnesota. But because PEDv may masquerade as a more benign pathogen, there's a crying need for a quick and definitive test.
In June 2013, just 12 weeks after being asked to help with the problem, a team at the University of Minnesota's Veterinary Diagnostic Laboratory (VDL) unveiled a rapid, reliable, and inexpensive test for the virus. While no cure exists, a positive test serves notice that a farm is infected so stronger biosecurity measures can be put in place.
It may not kill mature pigs, but even a farm with no piglets could suffer economically from PEDv.
"We don't know if there's a slowdown in growth—we've not seen one yet," says James Collins, director of the VDL. "That's the biggest threat to the swine industry.
"Or, it could enter a mature herd silently and then spread through manure. There isn't enough knowledge and biosecurity to control its spread."
To view the complete article, please visit the UMNews.
Elitest For Detection of Antibodies to Caprine Arthritis-Encephalitis Virus and Maedi-visna Virus (CAEV/MVV)
Devi P. Patnayak and Sagar M. Goyal
Veterinary Diagnostic Laboratory
University of Minnesota, College of Veterinary Medicine
Caprine arthritis encephalitis virus (CAEV) and Maedi-visna virus (MVV) are genetically and antigenically closely related viruses. Various commercial ELISA test kits are available for detection of antibodies to MVV/CAE viruses including: Idexx CAEV/MVV kit, Pourquier ELISA Maedi- visna/CAEV antibody test kit, Elitest MVV/CAEV Hyphen Biomed kit and VMRD small ruminant lentivirus antibody test kit. Another serological test commonly used is agar gel immunodiffusion (AGID).
The Veterinary Diagnostic Laboratory (VDL) at University of Minnesota currently offers VMRD small ruminant lentivirus antibody test kit for CAE/MVV serology. Elitest, available in Europe from Hyphen-Biomed, is an MVV ELISA based on recombinant protein and synthetic peptides and has been reported to have overall sensitivity of 99.4 % and specificity of 99.8% (Saman et al., 1999). We recently compared this kit with the VMRD kit using sheep and goat samples. A total of 71 samples tested by VMRD kit were tested by Elitest. The results obtained were as follows:
There were 15 samples having discrepant results between the two tests. All of these 15 samples were positive by the VMRD test and negative by Elitest. Six of these 15 samples were tested again by both tests and the results were the same.
A set of 8 samples tested by AGID were also tested by Elitest. The results of both tests were the same (3 of 8 samples positive).
The actual status of these samples is unknown. Based on our results, therefore, we cannot say with certainty if one test is better than the other. In a study conducted at the University of Guelph, different ELISA kits were evaluated and Elitest was found to have the best sensitivity.
Additonal information can be found at the uoguelph website.
The Minnesota VDL will start offering the Elitest starting October 21, 2013. This test was approved by Minnesota Board of Animal Health to be used in OPP Pilot Program. Ours will be the first lab to introduce and offer this test in the United States. We thank Londa Kroone for her help in this testing.
References: 1. Saman, E., Van Eynde, G., Lujan, L., Extramiana, B., Harkiss, G., Tolari, F., Gonzalez, L., Amorena, B., Watt, N., and Badiola, J. 1999. A new sensitive serological assay for detection of lentivirus infections in small ruminants. Clinical and Diagnostic Laboratory Immunology, 6: 734-740.
Each month in Animal Bytes, one piece of the new Board of Animal Health rules will be highlighted.
Except for swine in slaughter-only classes, breeding and feeding swine at public exhibitions must be officially identified.